N complete RPMI medium for 48 h SVF cell cultures or in RPMI - 1640 supplemented with 10 mM Hepes, 60 IU/ml penicillin, 60 g/ml streptomycin, 50 M 2-mercaptoethanol, and 10 FBS for SVF cell cultures to be used in flow cytometric analysis.Flow cytometric analysis. For cytokine intracellular staining, SVF cells (1 ?106cells per well) isolated as described above were incubated for 4 h 30 min at 37


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